# Cost-efficient whole genome-sequencing using novel mostly natural sequencing-by-synthesis chemistry and open fluidics platform

Source: https://onco.cc/key-papers/paper-almogy-biorxiv/  
OnCo record `paper-almogy-biorxiv` (Key paper). Data CC BY-NC 4.0, attribute "Data from OnCo (onco.cc)"; commercial use needs a licence.

## TL;DR

Preprint cited by one technology page, indexed on Europe PMC as preprint record PPR499502 and posted on bioRxiv; the citing page links this DOI, which is how the record was matched.

## Summary

We introduce a massively parallel novel sequencing platform that combines an open flow cell design on a circular wafer with a large surface area and mostly natural nucleotides that allow optical end-point detection without reversible terminators. This platform enables sequencing billions of reads with longer read length (∼300bp) and fast runs times (<20hrs) with high base accuracy (Q30 > 85%), at a low cost of $1/Gb. We establish system performance by whole-genome sequencing of the Genome-In-A-Bottle reference samples HG001-7, demonstrating high accuracy for SNPs (99.6%) and Indels in homopolymers up to length 10 (96.4%) across the vast majority (>98%) of the defined high-confidence regions of these samples. We demonstrate scalability of the whole-genome sequencing workflow by sequencing an additional 224 selected samples from the 1000 Genomes project achieving high concordance with reference data.

Indexed on Europe PMC as preprint record PPR499502 (DOI 10.1101/2022.05.29.493900). Matched by DOI alone: one technology page cites this DOI among its external links (the pages are listed under Related), and this page was written so that the citation resolves inside OnCo. No figure has been checked by an editor.

## Fields

- Kind: Key paper
- Last checked: 2026-09-22
- Tags: europepmc-ingest; preprint
- Journal: bioRxiv (preprint)
- Year: 2022
- DOI: 10.1101/2022.05.29.493900
- Authors: Almogy G, Pratt M, Oberstrass F, et al.
- What it means: One technology page on OnCo cites this preprint by its DOI; this record gives the citation a page of its own so a reader can follow it without leaving OnCo. Read the abstract above alongside the citing page listed under Related; the record was created automatically from the Europe PMC entry and its figures have not been checked by hand.
- Caveats: Matched to the citing OnCo records by DOI alone; the summary reproduces the Europe PMC abstract and no figure has been verified against the full paper.; This is a preprint: it has not been peer reviewed, and Europe PMC links no journal version of it at the time of writing.

## Sources

- bioRxiv 2022: https://doi.org/10.1101/2022.05.29.493900
- Europe PMC: https://europepmc.org/article/PPR/PPR499502

## Connected records

- technologies: [New short-read sequencing platforms](https://onco.cc/technologies/next-gen-short-read-platforms/)

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