# RFWD3

Source: https://onco.cc/targets/rfwd3/  
OnCo record `rfwd3` (Target). Data CC BY-NC 4.0, attribute "Data from OnCo (onco.cc)"; commercial use needs a licence.

## TL;DR

RFWD3 (E3 ubiquitin-protein ligase RFWD3) is an enzyme. The public catalogues list it as a DNA repair gene, and the evidence so far is association rather than a proven role.

## Summary

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signalling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination.

Open Targets scores its association with cancer at 0.51 (direct and indirect evidence; datatypes literature 0.89, genetic association 0.56, somatic mutation 0.49).

## Fields

- Kind: Target
- Last checked: 2026-09-23
- Also known as: ring finger and WD repeat domain 3; E3 ubiquitin-protein ligase RFWD3; FLJ10520; RNF201; FANCW
- Tags: cancer-genes-wave
- Symbol: RFWD3
- Class: enzyme
- Biology: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signalling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalysing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionising radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Location: Nucleus; Nucleus, PML body; Cytoplasm (UniProt). Locus 16q23.1 (HGNC).

## Notes

- Written by scripts/fetch-cancer-genes.ts from CIViC, Open Targets, IntOGen, HGNC and UniProt; the function text is UniProt's, condensed and in UK spelling. Roles: UniProt keyword "DNA repair". Evidence tier "association-only" is the strongest of those signals.
- Prevalence not recorded: none of the sources gives a positivity rate.

## Sources

- HGNC HGNC:25539: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:25539
- UniProt Q6PCD5: https://www.uniprot.org/uniprotkb/Q6PCD5/entry
- NCBI Gene 55159: https://www.ncbi.nlm.nih.gov/gene/55159
- Ensembl ENSG00000168411: https://www.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000168411

## Connected records

- collections: [Open Targets Platform](https://onco.cc/collections/open-targets/)

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JSON: https://onco.cc/api/v1/entities/rfwd3.json