{"entity":{"id":"paper-elyada-antigen-presenting-cafs-single-cell-cancer-discov-2019","kind":"paper","name":"Cross-species single-cell analysis of pancreatic ductal adenocarcinoma reveals antigen-presenting cancer-associated fibroblasts","aka":[],"tldr":"Reading pancreatic tumours one cell at a time confirmed the two known fibroblast types and found a third that carries the molecules used to show antigens to immune cells and can switch on helper T cells.","summary":"Single-cell RNA sequencing characterised the neoplastic and microenvironment content of human and mouse pancreatic tumours. Myofibroblastic and inflammatory CAFs were corroborated and their in vivo gene signatures defined. A new CAF population expressing MHC class II and CD74 but not classic costimulatory molecules was described and termed antigen-presenting CAFs; they activated CD4-positive T cells in an antigen-specific fashion in a model system, confirming an immune-modulatory capacity.","asOf":"2026-09-24","links":[{"label":"Elyada et al., Cancer Discov 2019: antigen-presenting fibroblasts by single-cell sequencing","url":"https://doi.org/10.1158/2159-8290.CD-19-0094"},{"label":"PubMed","url":"https://pubmed.ncbi.nlm.nih.gov/31197017/"}],"tags":[],"related":[],"cancers":["pancreatic"],"sections":[],"technologies":["single-cell-spatial"],"targets":[],"drugs":[],"companies":[],"institutions":["cold-spring-harbor"],"pathways":["caf-activation-desmoplasia","antigen-presentation-immunoediting","tumor-microenvironment"],"terms":[],"trials":[],"people":["david-tuveson","elizabeth-jaffee"],"bottlenecks":[],"keyPapers":[],"journals":["cancer-discovery"],"dependsOn":[],"notes":[],"journal":"Cancer Discovery","year":2019,"doi":"10.1158/2159-8290.CD-19-0094","pmid":"31197017","authors":"Elyada E, Bolisetty M, Laise P, et al.","paperType":"basic","findings":["Three CAF states: myofibroblastic, inflammatory and antigen-presenting.","Antigen-presenting CAFs express MHC class II and CD74 and activate CD4 T cells in a model."],"whatItMeans":"Fibroblasts are part of the immune conversation, not just a physical barrier, which is why stromal and immune strategies are now designed together.","caveats":["Descriptive single-cell atlas; function shown in a model system.","Whether antigen-presenting CAFs help or suppress immunity in patients is unknown."],"changedPractice":false},"route":"/key-papers/paper-elyada-antigen-presenting-cafs-single-cell-cancer-discov-2019/","neighbours":{"cancer":[{"id":"pancreatic","kind":"cancer","name":"Pancreatic ductal adenocarcinoma","route":"/cancers/pancreatic/"}],"technology":[{"id":"single-cell-spatial","kind":"technology","name":"Single-cell & spatial profiling","route":"/technologies/single-cell-spatial/"}],"institution":[{"id":"cold-spring-harbor","kind":"institution","name":"Cold Spring Harbor Laboratory","route":"/institutions/cold-spring-harbor/"}],"pathway":[{"id":"antigen-presentation-immunoediting","kind":"pathway","name":"Antigen presentation & immune editing","route":"/pathways/antigen-presentation-immunoediting/"},{"id":"caf-activation-desmoplasia","kind":"pathway","name":"Fibroblast activation, desmoplasia & matrix stiffness","route":"/pathways/caf-activation-desmoplasia/"},{"id":"tumor-microenvironment","kind":"pathway","name":"Tumour microenvironment (TME)","route":"/pathways/tumor-microenvironment/"}],"person":[{"id":"david-tuveson","kind":"person","name":"David A. Tuveson","route":"/people/david-tuveson/"},{"id":"elizabeth-jaffee","kind":"person","name":"Elizabeth M. Jaffee","route":"/people/elizabeth-jaffee/"}],"journal":[{"id":"cancer-discovery","kind":"journal","name":"Cancer Discovery","route":"/journals/cancer-discovery/"}],"term":[{"id":"caf-subtypes-pancreatic","kind":"term","name":"Fibroblast subtypes in the pancreatic cancer stroma (myCAF, iCAF and apCAF)","route":"/terms/caf-subtypes-pancreatic/"}]}}