{"entity":{"id":"paper-sun-foundationone-rna-validation-plos-one-2025","kind":"paper","name":"Analytical validation (accuracy, reproducibility, limit of detection) and gene expression analysis of FoundationOneRNA assay for fusion detection in 189 clinical tumor specimens","aka":[],"tldr":"The laboratory validation of Foundation Medicine's tumour RNA fusion test in 189 tumour samples.","summary":"FoundationOne RNA detects fusions in 318 genes and measures expression of 1,521 genes. Against orthogonal assays, positive percent agreement was 98.28 per cent and negative percent agreement 99.89 per cent; it found a low-level BRAF fusion that orthogonal whole-transcriptome sequencing missed.","asOf":"2026-11-04","links":[{"label":"Sun et al., PLoS One 2025","url":"https://doi.org/10.1371/journal.pone.0329697"},{"label":"PubMed","url":"https://pubmed.ncbi.nlm.nih.gov/40938919/"}],"tags":[],"related":[],"cancers":[],"sections":["diagnostics"],"technologies":["rna-seq","cgp"],"targets":[],"drugs":["foundationone-rna"],"companies":[],"institutions":[],"pathways":[],"terms":["biomarker"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":[],"journal":"PLoS One","year":2025,"doi":"10.1371/journal.pone.0329697","pmid":"40938919","authors":"Sun D, Huang RSP, Green M, et al.","paperType":"translational","findings":["Positive agreement 98.28%, negative agreement 99.89%."],"whatItMeans":"A targeted RNA test can supplement DNA profiling for fusions.","caveats":["Analytical validation by the maker; clinical benefit not tested."],"participants":189},"route":"/key-papers/paper-sun-foundationone-rna-validation-plos-one-2025/","neighbours":{"section":[{"id":"diagnostics","kind":"section","name":"Diagnostics & Biomarkers","route":"/fronts/diagnostics/"}],"technology":[{"id":"cgp","kind":"technology","name":"Comprehensive genomic profiling","route":"/technologies/cgp/"},{"id":"rna-seq","kind":"technology","name":"RNA sequencing & expression profiling","route":"/technologies/rna-seq/"}],"drug":[{"id":"foundationone-rna","kind":"drug","name":"FoundationOne RNA","route":"/drugs/foundationone-rna/"}],"term":[{"id":"biomarker","kind":"term","name":"Biomarker","route":"/terms/biomarker/"}]}}