{"entity":{"id":"paper-tsui-plasma-rna-stability-clin-chem-2002","kind":"paper","name":"Stability of endogenous and added RNA in blood specimens, serum, and plasma","aka":[],"tldr":"A laboratory study showing that the body's own RNA in plasma is protected and stable, while free RNA added to plasma is destroyed within seconds.","summary":"Plasma RNA concentrations from uncentrifuged blood left at 4 degrees for 0, 6 and 24 hours did not differ significantly (P = 0.182), and a single freeze-thaw cycle had no significant effect, while more than 99 per cent of free added RNA could no longer be amplified after 15 seconds in plasma.","asOf":"2026-11-04","links":[{"label":"Tsui et al., Clin Chem 2002","url":"https://doi.org/10.1093/clinchem/48.10.1647"},{"label":"PubMed","url":"https://pubmed.ncbi.nlm.nih.gov/12324479/"}],"tags":[],"related":[],"cancers":[],"sections":["diagnostics"],"technologies":["liquid-rna-biopsy"],"targets":[],"drugs":[],"companies":[],"institutions":[],"pathways":[],"terms":["biomarker"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":[],"journal":"Clinical Chemistry","year":2002,"doi":"10.1093/clinchem/48.10.1647","pmid":"12324479","authors":"Tsui NBY, Ng EKO, Lo YMD","paperType":"translational","findings":[">99% of added free RNA unamplifiable after 15 seconds in plasma.","Endogenous plasma RNA stable for 24 hours at 4 degrees in whole blood."],"whatItMeans":"Tumour RNA reaches a blood test only if it is packaged; how much of a tumour cell's RNA survives that way is unknown.","caveats":["Healthy volunteers; one housekeeping gene."],"participants":65},"route":"/key-papers/paper-tsui-plasma-rna-stability-clin-chem-2002/","neighbours":{"section":[{"id":"diagnostics","kind":"section","name":"Diagnostics & Biomarkers","route":"/fronts/diagnostics/"}],"technology":[{"id":"liquid-rna-biopsy","kind":"technology","name":"Liquid RNA biopsy (cell-free, vesicle and platelet RNA)","route":"/technologies/liquid-rna-biopsy/"}],"term":[{"id":"biomarker","kind":"term","name":"Biomarker","route":"/terms/biomarker/"}],"idea":[{"id":"idea-stack-ctdna-and-tumour-rna-residual-disease","kind":"idea","name":"Read tumour RNA and tumour DNA from the same blood draw to find and type residual cancer","route":"/ideas/idea-stack-ctdna-and-tumour-rna-residual-disease/"}]}}