{"entity":{"id":"paper-vorperian-cell-types-cell-free-transcriptome-nat-biotechnol-2022","kind":"paper","name":"Cell types of origin of the cell-free transcriptome","aka":[],"tldr":"A study that worked out which cell types the RNA in healthy people's blood comes from, finding most from platelets, red cell precursors and white cells.","summary":"Deconvolution of plasma cell-free RNA against a single-cell atlas found that platelets, erythrocyte and erythroid progenitors and leukocytes comprised the majority of observed signal. The authors note that the method assigns a reduced contribution to cell types that express genes at lower levels or are smaller.","asOf":"2026-11-04","links":[{"label":"Vorperian et al., Nat Biotechnol 2022","url":"https://doi.org/10.1038/s41587-021-01188-9"},{"label":"PubMed","url":"https://pubmed.ncbi.nlm.nih.gov/35132263/"}],"tags":[],"related":[],"cancers":[],"sections":["diagnostics"],"technologies":["liquid-rna-biopsy","single-cell-spatial"],"targets":[],"drugs":[],"companies":[],"institutions":[],"pathways":[],"terms":["biomarker"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":[],"journal":"Nature Biotechnology","year":2022,"doi":"10.1038/s41587-021-01188-9","pmid":"35132263","authors":"Vorperian SK, Moufarrej MN, Tabula Sapiens Consortium, Quake SR","paperType":"translational","findings":["Platelets, erythroid cells and leukocytes dominate plasma RNA (full text)."],"whatItMeans":"Any tumour RNA in blood is read against a large background from blood cells.","caveats":["Healthy plasma; no tumour fraction."]},"route":"/key-papers/paper-vorperian-cell-types-cell-free-transcriptome-nat-biotechnol-2022/","neighbours":{"section":[{"id":"diagnostics","kind":"section","name":"Diagnostics & Biomarkers","route":"/fronts/diagnostics/"}],"technology":[{"id":"liquid-rna-biopsy","kind":"technology","name":"Liquid RNA biopsy (cell-free, vesicle and platelet RNA)","route":"/technologies/liquid-rna-biopsy/"},{"id":"single-cell-spatial","kind":"technology","name":"Single-cell & spatial profiling","route":"/technologies/single-cell-spatial/"}],"term":[{"id":"biomarker","kind":"term","name":"Biomarker","route":"/terms/biomarker/"}]}}