{"entity":{"id":"paper-zheng-anchored-multiplex-pcr-nat-med-2014","kind":"paper","name":"Anchored multiplex PCR for targeted next-generation sequencing","aka":[],"tldr":"The paper that introduced anchored multiplex PCR, a way of finding gene fusions in tumour samples without knowing the partner gene.","summary":"Validation of a gene rearrangement panel on 319 fixed tumour samples showed 100 per cent sensitivity (95% confidence limit 96.5 to 100) and 100 per cent specificity against reference assays; the method found new fusions including ARHGEF2-NTRK1 in glioblastoma.","asOf":"2026-11-04","links":[{"label":"Zheng et al., Nat Med 2014","url":"https://doi.org/10.1038/nm.3729"},{"label":"PubMed","url":"https://pubmed.ncbi.nlm.nih.gov/25384085/"}],"tags":[],"related":[],"cancers":[],"sections":["diagnostics"],"technologies":["rna-seq"],"targets":[],"drugs":["archer-fusionplex"],"companies":[],"institutions":[],"pathways":[],"terms":["biomarker"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":[],"journal":"Nature Medicine","year":2014,"doi":"10.1038/nm.3729","pmid":"25384085","authors":"Zheng Z, Liebers M, Zhelyazkova B, et al.","paperType":"translational","findings":["100% sensitivity and specificity in 319 samples."],"whatItMeans":"The basis of the FusionPlex panels many laboratories use.","caveats":["Validation against reference assays in one centre."],"participants":319},"route":"/key-papers/paper-zheng-anchored-multiplex-pcr-nat-med-2014/","neighbours":{"section":[{"id":"diagnostics","kind":"section","name":"Diagnostics & Biomarkers","route":"/fronts/diagnostics/"}],"technology":[{"id":"rna-seq","kind":"technology","name":"RNA sequencing & expression profiling","route":"/technologies/rna-seq/"}],"drug":[{"id":"archer-fusionplex","kind":"drug","name":"Archer FusionPlex panels","route":"/drugs/archer-fusionplex/"}],"term":[{"id":"biomarker","kind":"term","name":"Biomarker","route":"/terms/biomarker/"}]}}