{"entity":{"id":"pathway-activation-state","kind":"term","name":"Pathway activation state (phosphosignalling)","aka":["pathway activation state","pathway activation","phosphosignalling","signalling activity","phosphorylation state"],"tldr":"Whether a signalling pathway is switched on is set by phosphorylation of its proteins, not by how much of them is present, so it has to be measured at the protein level.","summary":"Phosphoproteomics identifies and characterises proteins carrying phosphate groups, a reversible modification that regulates protein function, localisation and complex formation (Wikipedia). Kinase pathways such as PI3K/AKT and RAS/MAPK are read from phosphosites (AKT S473, ERK T202/Y204) by mass spectrometry or reverse-phase protein arrays; CPTAC's phosphoproteome and TCGA's RPPA are the public sources. Expression models infer activation indirectly through downstream transcriptional targets such as the SPRY and DUSP genes.","asOf":"2026-09-24","wikipedia":"https://en.wikipedia.org/wiki/Phosphoproteomics","links":[{"label":"Wikipedia","url":"https://en.wikipedia.org/wiki/Phosphoproteomics"}],"tags":["cansim-terms"],"related":["cancer-ai-vocabulary"],"cancers":[],"sections":[],"technologies":["proteomics"],"targets":[],"drugs":[],"companies":[],"institutions":[],"pathways":["pi3k-akt-mtor","ras-mapk"],"terms":["rppa","mrna-protein-concordance"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":["Listed in the CanSim terms map 1.0.0 (docs/onco/terms.json, generated 2026-09-24), CC BY 4.0, attribution: CanSim project, an open, public-data-first cancer foundation-model programme; CanSim page path /terms/pathway-activation-state."],"provenance":{"editedBy":"OnCo CanSim terms wave (Wikipedia summaries, standards and project pages, GDC and FDA pages, Europe PMC)","editedOn":"2026-09-24","note":"CanSim terms map 1.0.0 (docs/onco/terms.json, generated 2026-09-24), CC BY 4.0, attribution: CanSim project, an open, public-data-first cancer foundation-model programme"},"category":"Cancer biology"},"route":"/terms/pathway-activation-state/","neighbours":{"term":[{"id":"cancer-ai-vocabulary","kind":"term","name":"Cancer AI vocabulary (CanSim terms map)","route":"/terms/cancer-ai-vocabulary/"},{"id":"mass-spec-proteome","kind":"term","name":"Deep mass-spectrometry proteome (CPTAC)","route":"/terms/mass-spec-proteome/"},{"id":"gsea","kind":"term","name":"Gene set enrichment analysis (GSEA and ssGSEA)","route":"/terms/gsea/"},{"id":"mrna-protein-concordance","kind":"term","name":"mRNA to protein concordance","route":"/terms/mrna-protein-concordance/"},{"id":"post-transcriptional-regulation-term","kind":"term","name":"Post-transcriptional and post-translational regulation","route":"/terms/post-transcriptional-regulation-term/"},{"id":"rppa","kind":"term","name":"Reverse-phase protein array (RPPA)","route":"/terms/rppa/"}],"technology":[{"id":"proteomics","kind":"technology","name":"Proteomics & phosphoproteomics","route":"/technologies/proteomics/"}],"pathway":[{"id":"pi3k-akt-mtor","kind":"pathway","name":"PI3K / AKT / mTOR","route":"/pathways/pi3k-akt-mtor/"},{"id":"ras-mapk","kind":"pathway","name":"RAS / RAF / MEK / ERK (MAPK)","route":"/pathways/ras-mapk/"}],"target":[{"id":"phlpp2","kind":"target","name":"PHLPP2","route":"/targets/phlpp2/"},{"id":"spry4","kind":"target","name":"SPRY4","route":"/targets/spry4/"}]}}