{"entity":{"id":"rppa","kind":"term","name":"Reverse-phase protein array (RPPA)","aka":["RPPA","reverse-phase protein array","reverse phase protein array","protein array data","RPPA data"],"tldr":"RPPA spots tiny amounts of protein extract from many tumours onto a slide and probes them with antibodies, measuring a few hundred proteins and phosphoproteins in each sample at once.","summary":"A reverse phase protein lysate microarray is a dot-blot platform that measures protein expression in many samples simultaneously and quantitatively where high-quality antibodies exist (Wikipedia). TCGA ran RPPA on most tumours for roughly 150 to 200 proteins and phosphoproteins chosen for signalling pathways, which makes it the only pan-cancer protein data at scale, though a targeted rather than genome-wide view. Deep mass spectrometry from CPTAC covers thousands of proteins on fewer tumours.","asOf":"2026-09-24","wikipedia":"https://en.wikipedia.org/wiki/Reverse_phase_protein_lysate_microarray","links":[{"label":"Wikipedia","url":"https://en.wikipedia.org/wiki/Reverse_phase_protein_lysate_microarray"}],"tags":["cansim-terms"],"related":["tcga-gdc","cancer-ai-vocabulary"],"cancers":[],"sections":[],"technologies":["proteomics"],"targets":[],"drugs":[],"companies":[],"institutions":[],"pathways":[],"terms":["pathway-activation-state","mass-spec-proteome"],"trials":[],"people":[],"bottlenecks":[],"keyPapers":[],"journals":[],"dependsOn":[],"notes":["Listed in the CanSim terms map 1.0.0 (docs/onco/terms.json, generated 2026-09-24), CC BY 4.0, attribution: CanSim project, an open, public-data-first cancer foundation-model programme; CanSim page path /terms/rppa."],"provenance":{"editedBy":"OnCo CanSim terms wave (Wikipedia summaries, standards and project pages, GDC and FDA pages, Europe PMC)","editedOn":"2026-09-24","note":"CanSim terms map 1.0.0 (docs/onco/terms.json, generated 2026-09-24), CC BY 4.0, attribution: CanSim project, an open, public-data-first cancer foundation-model programme"},"category":"Biomarkers"},"route":"/terms/rppa/","neighbours":{"collection":[{"id":"tcga-gdc","kind":"collection","name":"TCGA / NCI Genomic Data Commons","route":"/collections/tcga-gdc/"}],"term":[{"id":"cancer-ai-vocabulary","kind":"term","name":"Cancer AI vocabulary (CanSim terms map)","route":"/terms/cancer-ai-vocabulary/"},{"id":"mass-spec-proteome","kind":"term","name":"Deep mass-spectrometry proteome (CPTAC)","route":"/terms/mass-spec-proteome/"},{"id":"pathway-activation-state","kind":"term","name":"Pathway activation state (phosphosignalling)","route":"/terms/pathway-activation-state/"}],"technology":[{"id":"proteomics","kind":"technology","name":"Proteomics & phosphoproteomics","route":"/technologies/proteomics/"}]}}