Make bespoke mouse cancer models in weeks with in vivo gene editing
Building a genetically engineered mouse for a specific cancer takes years. Editing genes directly in an adult mouse's organ can produce the same tumour in weeks.
Somatic genome editing by electroporation, viral delivery or lipid nanoparticles into a target organ produces autochthonous tumours with intact immune systems and native microenvironment, at a fraction of the time and cost of germline models. This has been demonstrated in lung, pancreas, liver and brain. Combinatorial guide libraries also allow genotype-response mapping in immunocompetent animals.
- Preclinical models that do not predict people · Nine in ten cancer drugs that work in mice fail in humans. Our models are the reason.
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not linked directly; found by shared links- InstitutionThe Jackson Laboratory Cancer Center
Shares Patient-derived xenografts, CRISPR functional genomics, Preclinical models that do not predict people.
- IdeaZebrafish avatars for a drug answer within a week
Shares Patient-derived xenografts, Preclinical models that do not predict people, Pancreatic ductal adenocarcinoma.
- IdeaBarcode patient-derived tumours to watch which clones win under each drug
Shares Patient-derived xenografts, CRISPR functional genomics, Preclinical models that do not predict people.
- IdeaMake in vivo metastasis screens a required step in drug discovery
Shares Patient-derived xenografts, CRISPR functional genomics, Preclinical models that do not predict people.
- IdeaEvery resistance mechanism found in a patient must be rebuilt in the laboratory
Shares CRISPR functional genomics, Preclinical models that do not predict people.
- IdeaPre-specified sample sizes for animal studies; no more 'representative' experiments
Shares Patient-derived xenografts, Preclinical models that do not predict people.
- IdeaBuild laboratory models of the organs cancer spreads to
Shares Patient-derived xenografts, Preclinical models that do not predict people.
- IdeaHumanised mice with an immune system matched to the tumour donor
Shares Patient-derived xenografts, Preclinical models that do not predict people.