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Five different stains for the same protein were run on 81 real lung cancer samples and read by pathologists from thirteen countries. Three of the five agree closely on tumour cells, one stains less and one stains more, and none of them can be read reliably on immune cells.
Eighty-one lung cancer specimens of various histological and sample types were stained with all five trial-validated PD-L1 assays (22C3, 28-8, SP142, SP263 and 73-10) and evaluated by an international panel of pathologists, with parallel assessment of digital images and of cytology cell blocks. The 22C3, 28-8 and SP263 assays gave highly comparable staining of tumour cells; SP142 was less sensitive and 73-10 more sensitive. Glass slide and digital scoring were highly concordant, with correlation above 0.96. Reliability between pathologists was very strong for tumour-cell scoring, with overall intraclass correlation 0.86 to 0.93, poor for immune-cell scoring at 0.18 to 0.19, and good for scoring on cytological cell block material at 0.78 to 0.85.
It is the reason a laboratory can run one stain and report against several drug labels, and the reason any decision resting on an immune-cell score rests on the least reproducible number in lung pathology.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), PD-L1 TPS (tumour proportion score), Journal of Thoracic Oncology.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC).
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation, Histopathology & immunohistochemistry.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), Histopathology & immunohistochemistry.
Shares PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation, Histopathology & immunohistochemistry.
Shares PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), PD-L1.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-1 / PD-L1 immune checkpoint & T-cell activation, PD-L1, Immune checkpoint inhibitors.