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Thirteen pathologists scored four different stains for the same protein on ninety lung cancers. They agreed well on the cancer cells and hardly at all on the immune cells, and one of the four stains consistently read lower than the others.
Four serial histological sections from 90 archival non-small-cell lung cancers were distributed to three sites, which performed immunohistochemistry with the 28-8 and 22C3 antibodies on the Dako Link 48 platform, SP142 on the Ventana Benchmark platform and E1L3N on the Leica Bond platform. Slides were scanned and scored by 13 pathologists estimating the percentage of malignant and immune cells expressing PD-L1. SP142 was an outlier with significantly lower mean scores in both compartments (tumour cells 1.99 against 2.96 for 22C3, 3.26 for 28-8 and 3.20 for E1L3N). Interassay concordance for tumour-cell scoring was high, 0.813, and low for immune-cell scoring, 0.277.
Together with the Blueprint work it establishes that a PD-L1 percentage reports the assay as much as it reports the tumour, so the threshold and the antibody have to be quoted together.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC).
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC).
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 IC score (immune-cell score, SP142), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation, Histopathology & immunohistochemistry.
Shares PD-L1 TC score (tumour-cell score, SP263 and 28-8), PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), Histopathology & immunohistochemistry.
Shares PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), PD-L1.
Shares PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), PD-1 / PD-L1 immune checkpoint & T-cell activation, Histopathology & immunohistochemistry.
Shares PD-L1 TPS (tumour proportion score), Immunohistochemistry (IHC), PD-L1.