Flow cytometry (immunophenotyping)
A machine that streams cells one by one past lasers and reads the surface proteins (CD markers) each carries, identifying what kind of cell it is. It is how leukaemias and lymphomas are typed and how residual disease is counted.
Antibodies tagged with fluorescent dyes label CD19, CD20, CD33, CD34, CD38, BCMA and dozens of other markers; the pattern (immunophenotype) distinguishes B from T from myeloid lineages and normal from malignant cells. It diagnoses acute leukaemia, CLL and myeloma from blood or marrow within hours, and multiparameter or next-generation flow measures minimal residual disease to one cell in 100,000-1,000,000, guiding transplant and maintenance decisions. It also confirms that a CAR-T target (CD19, BCMA) is still present after relapse, since antigen loss is a recognised escape.
Pages like this
not linked directly; found by shared links- InstitutionGIMEMA
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.
- TechnologyMultiparameter flow cytometry MRD
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.
- TermLeukaemia (tissue type)
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.
- InstitutionSylvester Comprehensive Cancer Center, University of Miami
Shares Minimal / molecular residual disease (MRD), Multiple myeloma, Acute myeloid leukaemia.
- CollectionLeukemia & Lymphoma Society (LLS)
Shares Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia, Acute myeloid leukaemia.
- TermAllogeneic stem cell transplant (allo-SCT)
Shares Undetectable MRD (uMRD / MRD-negative), Acute lymphoblastic leukaemia, Acute myeloid leukaemia.
- TermComplete response (CR) and partial response (PR)
Shares Blasts (leukaemic blast cells), Undetectable MRD (uMRD / MRD-negative).
- InstitutionEuropean Hematology Association
Shares Minimal / molecular residual disease (MRD), Chronic lymphocytic leukaemia, Multiple myeloma, Acute lymphoblastic leukaemia.