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Using routine formalin-fixed tissue from 309 operations, this European study confirmed the classical and basal-like tumour types, added three microenvironment-defined groups, and showed the earlier exocrine-like type was contamination by normal acinar cells.
Formalin-fixed paraffin-embedded samples from 309 consecutive resections at four European hospitals underwent gene expression, targeted sequencing and immunohistochemistry. Independent component analysis deconvoluted normal, tumour and microenvironment patterns; consensus clustering after removing normal contamination defined subtypes, associated with survival and validated in TCGA and ICGC. The basal-like and classical tumour-specific subtypes were validated and five subtypes were defined: pure basal-like, stroma activated, desmoplastic, pure classical and immune classical. An exocrine signal was associated with acinar cell contamination, and the previously reported exocrine-like (ADEX) subtype was attributed to it.
It made subtyping possible on the archived tissue every hospital has, and it retired one of the four Bailey subtypes.
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), Pancreatic ductal adenocarcinoma.
Shares Institut Jules Bordet, Tumour microenvironment (TME).
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), RNA sequencing & expression profiling, Pancreatic ductal adenocarcinoma.
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), Pancreatic ductal adenocarcinoma.
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), Pancreatic ductal adenocarcinoma.
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), Pancreatic ductal adenocarcinoma.
Shares Fibroblast activation, desmoplasia & matrix stiffness, Tumour microenvironment (TME), Pancreatic ductal adenocarcinoma.