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A way of reading both strands of each DNA molecule in a blood sample, so a real mutation can be told from a copying error even when only one molecule in 100,000 carries it.
SaferSeqS (Cohen et al., Nature Biotechnology 2021, Johns Hopkins) introduces identical molecular barcodes on the Watson and Crick strands of each template molecule and enriches targets with strand-specific PCR. It detects variants below 1 in 100,000 template molecules with a background mutation rate below 5 x 10^-7 mutants per base pair, can assess one or several amplicons, recovers both strands well from limited cell-free DNA, and cuts the error rate of existing barcoding approaches by more than 100-fold. It reaches that level per locus, for the few mutations it assays. Haystack MRD describes its chemistry as an optimised version of the earlier Safe-SeqS method from the same group.
A true mutation is present on both strands of a double-stranded molecule; an error made while copying is usually on one. Requiring both strands, tied together by barcodes, removes most errors.
Query for this technology: (TITLE:"SaferSeqS" OR ABSTRACT:"SaferSeqS" OR TITLE:"Watson and Crick strand sequencing" OR ABSTRACT:"Watson and Crick strand sequencing") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about SaferSeqS (Watson and Crick strand sequencing), not a curated reading list.
Shares Read methylation and mutation on the same cell-free DNA fragment to tell what kind of cancer is left, Circulating tumour DNA (ctDNA), Liquid biopsy (ctDNA).
Shares Haystack MRD, MRD / molecular residual disease testing, Liquid biopsy (ctDNA).
Shares Haystack MRD, Circulating tumour DNA (ctDNA), MRD / molecular residual disease testing, Liquid biopsy (ctDNA).
Shares Haystack MRD, Circulating tumour DNA (ctDNA), MRD / molecular residual disease testing.
Shares Read methylation and mutation on the same cell-free DNA fragment to tell what kind of cancer is left, Circulating tumour DNA (ctDNA), MRD / molecular residual disease testing.
Shares Haystack MRD, MRD / molecular residual disease testing.
Shares Read methylation and mutation on the same cell-free DNA fragment to tell what kind of cancer is left, Circulating tumour DNA (ctDNA), MRD / molecular residual disease testing.
Shares Read methylation and mutation on the same cell-free DNA fragment to tell what kind of cancer is left, Circulating tumour DNA (ctDNA), Liquid biopsy (ctDNA).
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