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RPPA spots tiny amounts of protein extract from many tumours onto a slide and probes them with antibodies, measuring a few hundred proteins and phosphoproteins in each sample at once.
A reverse phase protein lysate microarray is a dot-blot platform that measures protein expression in many samples simultaneously and quantitatively where high-quality antibodies exist (Wikipedia). TCGA ran RPPA on most tumours for roughly 150 to 200 proteins and phosphoproteins chosen for signalling pathways, which makes it the only pan-cancer protein data at scale, though a targeted rather than genome-wide view. Deep mass spectrometry from CPTAC covers thousands of proteins on fewer tumours.
Showing the technology this term belongs to: Proteomics & phosphoproteomics.
Shares Pathway activation state (phosphosignalling), Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares Pathway activation state (phosphosignalling), Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares Deep mass-spectrometry proteome (CPTAC), Pathway activation state (phosphosignalling), Proteomics & phosphoproteomics, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares Pathway activation state (phosphosignalling), Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares Pathway activation state (phosphosignalling), Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares TCGA / NCI Genomic Data Commons, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares TCGA / NCI Genomic Data Commons, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares TCGA / NCI Genomic Data Commons, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.