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Whether a signalling pathway is switched on is set by phosphorylation of its proteins, not by how much of them is present, so it has to be measured at the protein level.
Phosphoproteomics identifies and characterises proteins carrying phosphate groups, a reversible modification that regulates protein function, localisation and complex formation (Wikipedia). Kinase pathways such as PI3K/AKT and RAS/MAPK are read from phosphosites (AKT S473, ERK T202/Y204) by mass spectrometry or reverse-phase protein arrays; CPTAC's phosphoproteome and TCGA's RPPA are the public sources. Expression models infer activation indirectly through downstream transcriptional targets such as the SPRY and DUSP genes.
Showing the technology this term belongs to: Proteomics & phosphoproteomics.
Shares Gene set enrichment analysis (GSEA and ssGSEA), Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares SPRY4, PHLPP2, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares mRNA to protein concordance, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares mRNA to protein concordance, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares PI3K / AKT / mTOR, Cancer AI vocabulary (CanSim terms map), RAS / RAF / MEK / ERK (MAPK) and the tag cansim-terms.
Shares mRNA to protein concordance, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares mRNA to protein concordance, Cancer AI vocabulary (CanSim terms map) and the tag cansim-terms.
Shares Cancer AI vocabulary (CanSim terms map), RAS / RAF / MEK / ERK (MAPK) and the tag cansim-terms.