OnCo

Fibroblasts and the extracellular matrix

Tumours keep the body's repair cells in wound-healing mode forever. The scar they lay down squeezes vessels shut, walls out immune cells, and its very stiffness tells cancer cells to grow. Demolishing it made pancreatic cancer worse; retraining it is the new plan.

Diagram

Pick a product above a diagram to see the nodes it hits and the escape routes below the block. Hover or tap any node or arrow for what it is; every node opens its target, glossary entry or the pathway page. Violet boxes are druggable targets.

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Tumour: TGF-β, PDGF, Hh, IL-1 activates Fibroblast / stellate cellFibroblast / stellate cell activates myCAF (FAP, αSMA, collagen)Fibroblast / stellate cell activates iCAF (IL-6, CXCL12, LIF)myCAF (FAP, αSMA, collagen) activates Collagen, HA, LOX crosslinksCollagen, HA, LOX crosslinks activates Stiffness → FAK → YAP/TAZStiffness → FAK → YAP/TAZ activates Growth, EMT, chemoresistanceStiffness → FAK → YAP/TAZ activates Fibroblast / stellate cellCollagen, HA, LOX crosslinks activates Pressure: vessels collapsemyCAF (FAP, αSMA, collagen) activates T-cell exclusioniCAF (IL-6, CXCL12, LIF) activates T-cell exclusionHedgehog (SMO) paradox activates Fibroblast / stellate cellTumour: TGF-β, PDGF, Hh, IL-1Tumour: TGF-β, PDGF, Hh, …Fibroblast / stellate cellFibroblast / stellate cellmyCAF (FAP, αSMA, collagen): FAP (fibroblast activation protein) sits on the cancer-associated fibroblasts that scaffold more than 90% of epithelial cancers and is almost absent from normal adult tissue.myCAF (FAP, αSMA, collage…iCAF (IL-6, CXCL12, LIF): The signalling enzyme that tells marrow cells to make red cells and platelets; a single mutation (V617F) leaves it switched on in most myeloproliferative neoplasms.iCAF (IL-6, CXCL12, LIF)Collagen, HA, LOX crosslinksCollagen, HA, LOX crossli…Stiffness → FAK → YAP/TAZStiffness → FAK → YAP/TAZPressure: vessels collapsePressure: vessels collapseT-cell exclusion: PD-1 is a brake on T cells. Blocking it releases the immune system against the tumour and has cured some previously incurable cancers.T-cell exclusionGrowth, EMT, chemoresistanceGrowth, EMT, chemoresista…Hedgehog (SMO) paradox: The switch in the hedgehog developmental pathway that is stuck on in basal cell carcinoma and some medulloblastomas; three approved pills block it.Hedgehog (SMO) paradoxactivatesinhibitsdruggable target (click)hit by selected productescape route
Fibroblast activation, desmoplasia & matrix stiffnessTumours recruit the body's repair cells, fibroblasts, and keep them in wound-healing mode forever. The scar tissue they lay down (desmoplasia) squeezes blood vessels shut, walls out immune cells, stiffens the tissue in a way that itself tells cancer cells to grow, and is why pancreatic cancer is so hard to treat.

Builders hired to repair a wall who never stop: they pour concrete around the tumour until the roads are blocked (vessels), the police cannot get in (T cells), and the very hardness of the concrete tells the tenants to multiply. Demolishing the builders' work made things worse; the newer plan is to retrain them.

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Tumour cells activates CAFs (FAP+)CAFs (FAP+) activates Stiff ECM / desmoplasiaStiff ECM / desmoplasia inhibits Exhausted CD8 T cells (PD-1)Tumour cells activates Abnormal vessels (VEGF)Abnormal vessels (VEGF) inhibits Exhausted CD8 T cells (PD-1)Tumour cells activates TAMs (CSF1R, CD47 axis)TAMs (CSF1R, CD47 axis) inhibits Exhausted CD8 T cells (PD-1)MDSCs inhibits Exhausted CD8 T cells (PD-1)Tregs (CTLA-4) inhibits Exhausted CD8 T cells (PD-1)Exhausted CD8 T cells (PD-1) inhibits Tumour cellsNerves activates Tumour cellsCAFs (FAP+) activates Tumour cellsTumour cellsTumour cellsCAFs (FAP+): FAP (fibroblast activation protein) sits on the cancer-associated fibroblasts that scaffold more than 90% of epithelial cancers and is almost absent from normal adult tissue.CAFs (FAP+)TAMs (CSF1R, CD47 axis): CD47 is the 'don't eat me' signal: it binds SIRP-alpha on macrophages to stop them engulfing the cell, and over 90% of AML blasts and large B-cell lymphoma cells display it.TAMs (CSF1R, CD47 axis)MDSCsMDSCsTregs (CTLA-4): The first immune brake ever targeted for cancer; releasing it won a Nobel Prize and cures a fraction of melanomas.Tregs (CTLA-4)Exhausted CD8 T cells (PD-1): PD-1 is a brake on T cells. Blocking it releases the immune system against the tumour and has cured some previously incurable cancers.Exhausted CD8 T cells (PD…Abnormal vessels (VEGF): The signal tumours use to grow their own blood supply. Blocking it starves tumours and, surprisingly, helps immunotherapy work.Abnormal vessels (VEGF)Stiff ECM / desmoplasiaStiff ECM / desmoplasiaNervesNervesactivatesinhibitsdruggable target (click)hit by selected productescape route
Tumour microenvironment (TME)A tumour is not just cancer cells. It is a neighbourhood of fibroblasts, immune cells, blood vessels, nerves, and scaffolding that the cancer recruits and corrupts, and that decides whether drugs and immune cells can get in.

A castle under siege from the inside: the cancer conscripts the town's builders (fibroblasts) to raise walls, bribes the guards (macrophages) to look away, and diverts the water supply (vessels) so that reinforcements (T cells, drugs) never arrive.

Contact, stiffness, GPCRs activates MST1/2 → LATS1/2NF2 (Merlin) activates MST1/2 → LATS1/2MST1/2 → LATS1/2 inhibits YAP/TAZYAP/TAZ activates TEAD transcriptionTEAD transcription activates Growth, EMT, drug toleranceContact, stiffness, GPCRsContact, stiffness, GPCRsNF2 (Merlin): NF2 (Merlin) is a gene whose normal job is to hold cell growth in check. The public catalogues list it as a drug target, an oncogene driver, a tumour suppressor and a biomarker, and clinical evidence ties its variants…NF2 (Merlin)MST1/2 → LATS1/2MST1/2 → LATS1/2YAP/TAZYAP/TAZTEAD transcriptionTEAD transcriptionGrowth, EMT, drug toleranceGrowth, EMT, drug toleran…activatesinhibitsdruggable target (click)hit by selected productescape route
Hippo-YAP/TAZThe pathway that tells organs when to stop growing. Cancers disable it so YAP and TAZ stay in the nucleus driving growth; in mesothelioma, NF2 loss does exactly that, and the first drugs against the YAP-TEAD switch are in trials.

A building inspector (Hippo) who checks that the block is full and stops new floors. Cancers fire the inspector, and the architect (YAP/TAZ) keeps adding storeys.

What happens

In plain words, then the glossary entries the stage rests on. Chapter 8, The tumour ecosystem: A tumour is a corrupted organ: cancer cells plus the fibroblasts, matrix, vessels, nerves, microbes and immune cells they recruit, and the signals they send to the rest of the body.

Tumours keep the body's repair cells in wound-healing mode forever. The scar they lay down squeezes vessels shut, walls out immune cells, and its very stiffness tells cancer cells to grow. Demolishing it made pancreatic cancer worse; retraining it is the new plan.

Fibroblast activation, desmoplasia & matrix stiffness. Tumours recruit the body's repair cells, fibroblasts, and keep them in wound-healing mode forever. The scar tissue they lay down (desmoplasia) squeezes blood vessels shut, walls out immune cells, stiffens the tissue in a way that itself tells cancer cells to grow, and is why pancreatic cancer is so hard to treat.

Tumour microenvironment (TME). A tumour is not just cancer cells. It is a neighbourhood of fibroblasts, immune cells, blood vessels, nerves, and scaffolding that the cancer recruits and corrupts, and that decides whether drugs and immune cells can get in.

Hippo-YAP/TAZ. The pathway that tells organs when to stop growing. Cancers disable it so YAP and TAZ stay in the nucleus driving growth; in mesothelioma, NF2 loss does exactly that, and the first drugs against the YAP-TEAD switch are in trials.

The molecular players

The proteins and genes at this stage, with their role and how many products act on each. Listed players come from the atlas; drawn players sit as nodes in the diagrams above.

  • The switch in the hedgehog developmental pathway that is stuck on in basal cell carcinoma and some medulloblastomas; three approved pills block it.

  • FAP (fibroblast activation protein) sits on the cancer-associated fibroblasts that scaffold more than 90% of epithelial cancers and is almost absent from normal adult tissue. FAPI PET tracers therefore light up tumours with high contrast, including pancreatic, gastric and low-grade cancers where FDG PET is weak, and FAP-targeted radioligands are in development.

  • PD-1 is a brake on T cells. Blocking it releases the immune system against the tumour and has cured some previously incurable cancers.

  • The signal tumours use to grow their own blood supply. Blocking it starves tumours and, surprisingly, helps immunotherapy work.

  • The first immune brake ever targeted for cancer; releasing it won a Nobel Prize and cures a fraction of melanomas.

  • The signalling enzyme that tells marrow cells to make red cells and platelets; a single mutation (V617F) leaves it switched on in most myeloproliferative neoplasms.

  • CD47 is the 'don't eat me' signal: it binds SIRP-alpha on macrophages to stop them engulfing the cell, and over 90% of AML blasts and large B-cell lymphoma cells display it. Blocking it should let macrophages eat tumour cells, but red cells carry CD47 too, so anaemia is built in, and the lead antibody magrolimab was dropped after failed trials.

Where medicines act

Products grouped by the node they hit, most advanced first, with the cancers an approved product is linked to. Pick one above the diagram to see it light up.

AtFAPnode myCAF (FAP, αSMA, collagen), CAFs (FAP+) in Fibroblast activation, desmoplasia & matrix stiffness and Tumour microenvironment (TME)2 products
AtCD47node TAMs (CSF1R, CD47 axis) in Tumour microenvironment (TME)3 products

How tumours escape

Records tied to this stage that describe resistance, evasion or tolerance. The resistance atlas lists the routes class by class.

Measured by

Biomarkers, tests and assays in the corpus that read this stage in a patient.

Open questions

What is not known at this stage: the atlas's own questions, the bottlenecks it bears on, and the ideas in the corpus that try to answer them.

  • Which fibroblast subtypes protect the tumour and which restrain it?
  • Can FAP radioligands treat stroma-rich cancers without harming healing tissue?
Ideas 33 linked ideas

23 more ideas are linked to this stage's pathways, targets and terms; see the rankings →

Key evidence

Papers in the corpus tied to this stage's pathways, targets and terms, newest first.

How this page is built: the stage is one entry in a curated atlas (src/data/mechanics-atlas.ts). Players, medicines, escape routes, tests, ideas and papers are resolved from the knowledge graph at build time through the stage's pathways, targets and terms, so every item here has its own page and sources. Where a section is missing, the corpus has no record tied to the stage yet. Nothing here is medical advice; see about and methodology. Stage 8.1 of 56.